大孔树脂吸附法对黄芪皂苷Ⅳ富集纯化工艺的研究

Study on enrichment and purification of astragalosides Ⅳ from Radix astragali with macroporous resins

目的:进行黄芪药材中黄芪皂苷Ⅳ的富集纯化工艺研究,为大规模制备黄芪皂苷Ⅳ提供借鉴。方法:建立高效液相色谱-质谱检测器(HPLC-MSD)测定黄芪皂苷Ⅳ含量的方法。对多种不同型号大孔树脂的吸附能力进行了筛选,同时考察确定了其吸附容量;通过对不同比例的乙醇溶液进行最佳洗脱液和洗脱量的选择。结果:HPLC-MSD测定方法学考察结果良好。工艺筛选最终确定吸附能力最强的D101型作为纯化工艺的大孔树脂,同时确定黄芪皂苷Ⅳ的吸附容量为0.42 mg/g,洗脱液为5倍量70%乙醇。结论:D101型大孔树脂对黄芪皂苷Ⅳ的精制程度效果较好,能提高黄芪皂苷Ⅳ的纯度,并为分析总皂苷的组成提供保证。

Objective： To study the enrichment and purification method of astragaloside Ⅳ from Radix astragali with macroporous resin, so as to lay a foundation for the large scale production of astragaloside Ⅳ. Methods： A HPLC-MSD method was established to determine the content of astragaloside Ⅳ in Radix astragali. The adsorption ability and capability of different macroporous resins were investigated. The optimal alcohol concentration and capability of alcohol for elution were also confirmed through the determination of astragaloside Ⅳ in the eluted solution. Results： HPLC-MSD achieved satisfactory results in determination of astragaloside Ⅳ. D101 was proven to be the most potent macroporous resinl its adsorption capability of astragaloside Ⅳwas 0.42 mg/g when the elution solution was 5 folds of 70% alcohol . Conclusion.. D101 macroporous resin can be used to effectively enrich and purify astragaloside Ⅳ, laying a foundation for analyzing the constituents of total astragalosides.