痹康饮合剂对佐剂性关节炎大鼠的实验研究

Experimental study of effect of Bikangyin Mixture on rats with adjuvant arthritis

目的探讨痹康饮合剂治疗类风湿性关节炎作用机制,为其临床治疗提供理论依据。方法建立佐剂诱导关节炎大鼠模型,通过关节炎指数评价、继发病变关节X线放射学和病理学检查、血清细胞因子测定,观察痹康饮合剂、甲氨喋呤对促炎症细胞因子IL-1β、TNF-α的调控作用。结果痹康饮合剂、甲氨喋呤治疗后,关节炎指数发生较显著的变化,与模型组相比有显著性差异(P<0.05),病理改变程度痹康饮合剂组最小。各组血清中细胞因子水平比较,对照组与各组IL-1β、TNF-α的水平比较有明显差异(P<0.05),痹康饮合剂组、甲氨喋呤组水平大致相同(P>0.05)。结论IL-1β、TNF-α异常增高与类风湿性关节炎发病密切相关,痹康饮合剂治疗可以降低佐剂性关节炎大鼠血清中异常增高的IL-1β、TNF-α浓度,从而抑制或控制类风湿性关节炎病情发展。

Objective To investigate the mechanism of the effect of Bikangyin Mixture on rheumatoid arthritis and provide the theoretical basis for clinical practice. Methods To establish the model of arthritis induced by adjuvant and observe the regulative effects of Bikangyin Mixture and methotrexate respectively on interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α） through the evaluation of arthritis index, X-ray radiological and pathological examination on secondary arthritis and the detection of serum cytokines. Result After the treatment with Bikangyin Mixture and methotrexate the arthritis index changed obviously with a significant difference compared with the model group （P 〈 0.05 ）. The order of pathological change degree from high to low was as follows： the model group, methotrexate group and Bikangyin Mixture group. There was a significant difference （P 〈 0.05） in the levels of IL-1β and TNF-α between the control group and other groups, while there was no significant difference between Bikangyin Mixture group and methotrexate group （P 〉 0.05）. Conclusion The abnormal increase of IL-β and TNF-α was related closely to rheumatoid arthritis. Bikangyin Mixture could decrease the concentrations of serum IL-1β and TNF-α level and control or inhibit the development of rheumatoid arthritis.