摘要
分别对不同继代次数（3～90代）‘富士’、‘金冠’、‘乔纳金’苹果组培苗进行POX、EST、AMY同工酶分析。结果表明，同一品种不同继代次数处理间均有一致的酶带谱型。利用超薄层等电聚焦电泳根据POX酶蛋白pI的差异可区分金冠和乔纳金两品种，同一品种不同继代次数的组培苗之间pI相同，组培过程未对POX酶蛋白pI产生影响。利用25个随机引物对不同继代次数的苹果组培继代苗进行了RAPD分析，其特征带型表现一致，未发现变异。说明苹果茎尖离体继代培养90次以内具有较高的遗传稳定性。
The genetic stability of apple plantlets repeatedly subcuhured in vitro for three to ninety times was examined by analysis of isozyme patterns of POX, EST and AMY, as well as RAPD. The results showed that there were clear difference in POX, EST, and AMY isozyme patterns among varieties, but there was no detetable difference in the same varieties with different times of subculturing. The POX isozyme was further analyzed by using IEF electrophoresis. It was easy to distinguish between genotypes （i. e. ＇ Golden Delicious＇ vs. ＇ Jonagold＇ ） according to the pI of POX isozyme. However, no difference was found in the same varieties with different times of subcuhuring. RAPD analysis with twenty-five 10-mer arbitrary primers revealed identical bands for plantlets with different times of subculturing within a variety. Our results indicated that genetic stability of apple plantlets was maintained even after repeatedly subcultured in vitro for up to ninety times.
出处
《园艺学报》
CAS
CSCD
北大核心
2006年第1期33-37,共5页
Acta Horticulturae Sinica
基金
河北省自然科学基金项目(C2004000368)
河北农业大学博士
引进人才启动基金资助项目
