梅PGIP基因的克隆及全序列分析

Cloning and Sequencing of PGIP Gene from Prunus mume Sieb

通过PCR扩增,从梅基因组中得到1条全长1 192 bp的多聚半乳糖醛酸酶抑制蛋白(PG IP)基因序列。该序列包含有1个完整的开放阅读框和1个内元。比对结果表明,克隆到的序列与桃、马哈利樱桃中相应序列一致度分别为96%和95%,其蛋白质序列与桃、马哈利樱桃的蛋白质序列一致度分别为97%和94%。该蛋白质序列中包含着一段亮氨酸重复序列。

A 1 192 bp sequence of polygalacturonase-inhibiting protein （PGIP） gene （Genbank locus： AY764131） was isolated by polymerase chain reaction （PCR） from Prunus mume. This sequence had a intron and a full open reading frame encoding the polygalacturonase-inhibiting protein. Its gene and derived protein were both highly homologous to those from Prunus persica and Prunus mahaleb. A conserved leucinerich fragment had existed in the derived protein sequence.