转rol基因枳橙分子鉴定及部分生物学的观测

Molecular Analysis,Morphological and Physiological Evaluation of the Transgenic Citrange Plants With rolA,rolB,rolC Genes

对转rol基因枳橙B、D、E3个系及对照田间高接植株进行了PCR分子鉴定、RT-PCR转基因表达分析和生物学特征的观测。PCR分析表明rolA、rolB、roIC已成功转入到3个转化枳橙系中。利用RT-PCR在田间转化植株中只检测到npt Ⅱ和rolC基因的表达，而rolA、rolB基因未检测到。3个转化系枳橙均表现出顶端优势减弱，侧枝增多，植株矮化性状明显，高度只有对照植株的44％～50％，节间长度缩短，叶面积减少；光合作用增强；内源赤霉素、生长素、玉米素等促进类激素在中部、下部叶片含量均高于对照，这可能是诱导上述形态特征的变化的因素之一。

Molecular identification, physiology evaluation and morphological observation of the rolA, rolB and rolC transgenic citrange clones （B, D, E） were conducted. PCR analysis indicated that the rolA, rolB, rolC genes were integrated into the citrange genome. The expression of the transgenes npt Ⅱ and rolC was detected by using RT-PCR; while the transgenes rolA and rolB did not show any RT-PCR product, and it was not clear for the factors related to their expression. In comparison with the control plants, the rol transgenic plant showed evident dwarfing characteristics, such as only half of the control in plant height, shorter internodal length, weaker apical dominance with more lateral branches, smaller leaf area, and higher net photosynthesis rate. Higher contents of the endogenous GA3, IAA and zeatin were found in the middle and basal parts of the transgenic shoots than the control, this could be one of the factors responsible for the above described morphological modifications.