金属离子对霍山石斛类原球茎增殖及植株再生的影响

Effects of Metal Ions on Protocorm-like Body Proliferation in Liquid Culture of Dendrobium huoshanense and Plantlet Regeneration

采用单因素浓度试验和正交试验研究了Mg2+、Ca2+、Fe2+、Mn2+和Zn2+离子对液体培养霍山石斛类原球茎增殖的影响。结果表明,5种金属离子对类原球茎增殖影响的主次顺序为Fe2+>Mn2+>Mg2+>Ca2+>Zn2+,促进类原球茎增殖的优化组合为Mg2+1.5 mmol.L-1、Ca2+4.5 mmol.L-1、Fe2+0.1mmol.L-1、Mn2+0.5 mmol.L-1、Zn2+0.06 mmol.L-1。经优化的液体培养基增殖的类原球茎能保持植株再生能力,平均每克类原球茎增殖后可再生植株1 985.2株,是相同时间内对照类原球茎再生植株数的4.2倍。

This research is concerned with optimization of Mg^2＋ , Ca^2＋ , Fe^2＋ , Mn^2＋ and Zn^2＋ for protocorm-like body proliferation in liquid culture of Dendrobium huoshanense by one factor-at-a-time and orthogohal matrix methods. Results showed that, except for Zn^^2＋ , the effects of other metal ions on PLB proliferation were significant and the order was Fe^2＋ 〉 Mn^2＋ 〉 Mg^2＋ 〉 Ca^2＋ 〉 Zn^2＋ The optimal concentration combination for PLB proliferation was determined as 1.5 mmol· L^-1 Mg^2＋ , 4. 5 mmol· L^-1 Ca2. , 0. 1 mmol· L^-1 Fe^2＋, 0. 5 mmol· L^-1 Mn^2＋ and 0. 06 mmol· L^-1 Zn^2＋. Proliferated PLBs remained the potential of plant regeneration. The amount of regenerated plantlets from per gram PLBs through proliferation in optimized liquid medium was 1 985.2 and was about 4. 2 times that from PLBs that proliferated on original medium.