深静脉血栓形成患者凝血因子Ⅷ Val34Leu基因多态性检测

Detection of Val34Leu polymorphism in factor Ⅷ gene in patients with deep venous thrombosis

目的探讨凝血因子X(FX)Val34Leu基因多态性与中国人群深静脉血栓形成(DVT)发病的相关性。方法利用聚合酶链反应和限制性片段长度多态性(PCR-RFLP)方法,检测了103例DVT患者与106例正常对照的FXVal34Leu基因多态,并加以对照分析。结果103例DVT患者FXVal34Leu基因型均为野生型V/V,未见突变类型;106例对照组中发现1例突变杂合子V/L。2组基因型频率、等位基因频率相互比较,差异均无统计学意义(P>0.05)。结论FXVal34Leu对中国人群DVT有保护作用的可能性极小。

ⅧVa134Leu ; thrombosis ; etiology ; gene polymorphism Abstract Aim： To investigate the role of polymorphism in the factor Ⅷ A-subunit gene （ⅧVal34Leu） for patients with deep venous thrombosis （DVT）. Methods： A total of 103 cases of DVT and 106 cases of normal control were detected the ⅧVa134Leu polymorphism by a combination of polymerase chain reaction-restriction fragment length polymorphism. Results： FⅧVal34Leu was detected in 0 of the patients with DVT and in 0.9% （1/106） of normal control. The genotype frequency and allele frequency in the 2 groups had no significant difference （ P 〉 0.05 ）. Conolusion ： The FⅧ Val34Leu polymorphism has little protective effect on DVT in Chinese.