膀胱癌患者细胞色素氧化酶P450 2C19基因多态性分析

Polymorphism of CYP2C19 gene in patients with bladder cancer

目的探讨膀胱癌患者细胞色素氧化酶P4502C19(CYP2C19)基因多态性。方法采用PCR-RFLP基因分析技术,对108例膀胱癌患者和112例非肿瘤同期住院对照患者CYP2C19基因2个常见突变位点m1和m2的多态性进行检测,并分析其与膀胱癌易感性的关系。结果与对照组相比,膀胱癌组中强代谢型(EMs)频率高于弱代谢型(PMs)(χ2=4.508,P<0.05)。吸烟、职业暴露、嗜食肉食为膀胱癌发病的危险因素。CYP2C19EMs与吸烟、职业暴露和嗜食肉食3个环境因素存在基因-环境交互作用,影响膀胱癌的发病。结论CYP2C19EMs基因型与膀胱癌易感性有关。

Aim： To investigate the genetic polymorphism of CYP2C19 in patients with bladder cancer （BC）. Methods： A total of 108 eases and 112 controls were enrolled to detect the CYP2C19 genotyping at two major polymorphie loci of gene coding area： CYP2C19ml and CYP2C19m2 using PCR-RFLP method. Results： Compared with control, the pereentage of EMs in BC group was higher than that of PMs （ P 〈 0.05 ）. Smoking, occupational exposure and addicted to meat consumption were risk factors to BC. The gene-environment interaction between CYP2C19 EMs and smoking, occupational exposure and addicted to meat consumption could increase the risk of BC signifieantly. Conclusion： There is association between polymorphism of CYP2C19 EMs gene and BC risk.