煤工尘肺患者血清转化生长因子β1、成纤维细胞生长因子含量检测

Determination of serum TGF-β1 and FGF of coal workers with pneumoconiosis

目的探讨转化生长因子(TGF-β1)和成纤维细胞生长因子(FGF)在煤工尘肺发生发展中的意义。方法分别采用双抗体夹心ELISA法、ABC-ELISA法测定50例煤工尘肺患者和50例健康对照人群血清TGF-β1、FGF的含量。结果煤工尘肺患者血清TGF-β1、FGF的含量分别为(60.24±17.57)μg/L、(209.72±59.71)ng/L,高于对照组的(42.81±11.01)μg/L和(77.58±52.82)ng/L。Ⅰ期煤工尘肺患者2指标血清水平与Ⅱ、Ⅲ期患者比较,差异无统计学意义(P>0.05)。尘肺患者血清TGF-β1与FGF水平呈正相关(r=0.419,P<0.05)。结论TGF-β1和FGF可能与煤工尘肺的发生发展有关。

Aim： To study the significance of TGF-β1 and FGF in the occurrence and development of coal workers＇ pneumoconiosis. Methods： The serum level of TGF-β1 and FGF of 50 patients suffering from coal workers＇pneumoconiosis and 50 healthy controls were determined using ELISA and ABC-ELISA, respectively. Results： The serum levels of TGF-β1 and FGF of patients were （60.24 ± 17.57） μg/L, （209.72 ± 59.71 ） ng/L, respectively, which were higher than those of the control （42.81 ± 11.01 ） μg/L and （77.58 ± 52.82 ） ng/L. There was no statistical significance between the first stage ,and the second and third stage patients in the serum levels of TGF-β1 and FGF. The serum levels of the two proteins of the patients were positively correlated （r = 0. 419,P 〈 0.05 ）. Conclusion： TGF-β1 and FGF may be related to the occurrence and development of coal workers＇pneumoconiosis.