微生物产生的冷休克蛋白研究进展

Advance in Cold Shock Proteins Produced by Microbes

冷休克蛋白(cold shock protein,Csp)首先在大肠杆菌中发现,它与微生物对冷环境的适应及多种细胞功能有关。冷休克蛋白基因是一段编码70个左右氨基酸的DNA序列,在这段序列中有5′非翻译区(5′UTR)、冷盒及下游盒等特征。冷休克蛋白作为DNA或RNA结合蛋白在基因表达调控过程中起重要作用。冷休克蛋白在转录、mRNA稳定性及翻译等几个水平上被严格调控。

The cold shock proteins （Csps） was first found in E. coli, and it functions as DNA and RNA binding protein, and also responsible for the cell to adapt the low temperature. Csps is a low molecular protein consist of 70 amino acids. CspA is the major cold shock protein in E. coli, and CspB in Bacillus subtilis. Both of their 8pace structures have been determined. The genes for Csp have some common characteristics, such as 5＇untranslated regions （5＇UTR）, cold box and downstream box. The production of Csp were controlled at the level of transcription, mRNA stability and translation.