高纯度茶皂素的分离

Preparation of high pure tea saponins

以经过处理的茶籽饼乙醇提取物为原料,考察了AB-8、H-103、AAS、D-4020、D-4006、NKA-9、S-8、X-5大孔吸附树脂对茶皂素的吸附效果和解析情况,确定AB-8树脂对茶皂素的吸附和洗脱效果较理想。利用AB-8大孔吸附树脂分离、丙酮沉淀、甲醇结晶的方法可将茶籽饼乙醇提取物中的茶皂素提纯到95%以上,得率大于50%。

With the material of pretreated ethanol extract of tea seed cake, the adsorption and desorption effects on tea saponins of macroporous adsorption resins were studied, such as AB - 8, H - 103, AAS, D - 4020, D - 4006, NKA - 9, S- 8, X- 5. The results showed that AB- 8 was the suitable resin of purifing tea saponins. The ethanol extract was treated by AB - 8 resin, then acetone precipitating and crystallization with 75% methanol were used to purify the tea saponins, and the purity of obtained tea saponins was over 95 %, and the yield was over 50%.