肺癌中FH IT基因缺失和p53蛋白表达的研究

Study on Deletion of Fragile Histidine Triad Gene and Expression of p53 Protein in Lung Cancer

目的:探讨肺癌组织中FH IT(frag ile h istid ine triad gene)基因缺失和p53蛋白表达及其与临床病理因素的关系。方法:采用逆转录-巢式聚合酶链反应方法及免疫组织化学ABC法分别检测42例肺癌组织中FH IT基因缺失和p53蛋白表达。结果:肺癌组织中FH IT基因缺失和p53蛋白表达阳性率分别为66.7%(28/42)和76.2%(32/42),FH IT基因缺失肺癌的p53蛋白阳性率明显高于FH IT基因正常表达的肺癌(P<0.01)。FH IT基因缺失与肺癌的淋巴结转移有关,p53蛋白表达与临床分期、淋巴结转移密切相关。结论:FH IT基因缺失和p53蛋白异常表达与肺癌发生、发展密切相关,二者相互促进,呈明显正相关。

Objective：To explore the relationship of fragile histidine triad gene （FHIT） deletion and expression of p53 protein to each other as well as to clinicopathological parameters in lung carcinomas. Methods：RT-PCR and immunohistoehemical method were adopted to detect respectively abnormalities of FHIT gene and expression of p53 protein in 42 cases of lung carcinomas. Results：The total positive rate of deletion of FHIT gene and expression of p53 protein was 66.70/00（28/42） and 76.20//00（32/42）,respectively in lung carcinomas. The positive rate of p53 protein in tumors of deletion of FHIT gene was significantly higher than in those of normal FHIT gene （P〈0. 01）. The deletion of FHIT gene in lung carcinomas was significantly correlated with lymph node metastasis ,while the expression of p53 protein was correlated with histological type, clinical Stages：and lymph node metastasis. Conclusion：The deletion of FHIT gene and expression of p53 protein in lung carcinomas relate to the occurrence and progression of tumors,and there is a significantly positive correlation between the deletion of FHIT gene and expression of p53 protein.