摘要
采用蛋白质双向电泳和图像分析技术,研究发现不同品种的家蚕5龄第4天后部丝腺细胞的蛋白质组成存在明显差异,并且蚕茧高产品种与蚕茧中、低产量品种之间所涉及的差异蛋白质斑点各不相同,暗示丝素基因的转录、翻译可能涉及了一个非常复杂的多点控制的调控系统,即不同品种的产茧量可能涉及不同的调控位点或调控因子。
Using two-dimensional electrophoresis of protein and image analysis system, we discovered that protein constituents in the posterior silkgland on 4^th day in 5^th instar are apparently different for different silkworm varieties. Comparing with those in silkworm variety with the middle, low yield cocoon, we found that there existed many different protein spots in silkworm variety with the high yeild cocoon. This suggested that the transcription and translation of fibroin gene might be involved in a complex regulation system with a great deal of regulation points, and the yeild cocoon in different varieties may be related with different regulation points or factors.
出处
《蚕业科学》
CAS
CSCD
北大核心
2006年第1期114-117,共4页
ACTA SERICOLOGICA SINICA
基金
国家重点基础研究发展计划973项目(编号2005CB121003)
浙江省科技厅项目(编号2004C32048)
关键词
家蚕
后部丝腺
蛋白质
双向电泳
图谱分析
Bombyx moti
Posterior silkgland
Protein
2D-PAGE
Image analysis