STZ－实验性糖尿病大鼠肾组织氧自由基与血浆6－酮前列腺素F_（1α）和血栓素B_2的关系

THE RELATIONSHIP BETWEEN OXYGEN FREE RADICALS OF TISSUE AND PLASMA 6-Keto-PGF1a TXB2 IN STZ-INDUCED DIABETIC RATS

目的：研究糖尿病早期高滤过状态与前列腺素及氧自由基生成之间的关系，以探求发病机理。方法：采用低温ＥＳＲ技术动态检测大鼠肾组织的氧自由基水平，同时用ＲＩＡ试剂盒测定血浆中６－酮前列腺素Ｆ＿（１α）（６－ｋｅｔｏ＋ＰＧＦ＿（１α））和血栓素Ｂ＿２（ＴＸＢ＿２）含量。对ＳＴＺ诱导的糖尿病大鼠模型，分别在造模后１、２、３、７、１４和２８ｄ测量上述指标。同时与对照组相比较。用ＰＲＩＭＥＲ程序完成数据的ｔ－检验和相关分析。结果：证实糖尿病肾组织氧水平及血浆６－ｋｅｔｏ－ＰＧＦ＿（１α）含量均较正常对照组明显增高（ｐ＜０．０５），并且糖尿病大鼠肾组织氧自由基水平增加与６－ｋｅｔｏ－ＰＧＦ＿（１α）／ＴＸＢ＿２呈显著正相关（ｒ＝０．８７９６）。结论：糖尿病早期肾组织氧自由基水平增加可能是导致血浆中６－ｋｅｔｏ－ＰＧＦ＿（１α）增加的原因之一，并且两者在糖尿病早期肾小球血流动力学改变过程中起某种协同作用。

Objective :Studying the relationship of the hyperfiltration of the nephrons and plasma prostaglandins and renal active oxygen free readlcals ln the early stage of diabetic nephropathy for clari-fying the pathogenic molecular mechanism.Metbods:Using low temperature dynamic ESR technique,the oxygen free radical as well tqs 6-Keto-PGF_(1α) and TXB_2 contents with RIA text-boxes,has been de-tected.Measurements to STZ-induced diabetic rat renals and controls have been finished on the lst.2nd,3rd,7th,4th and 28th day after modeling,respectively.Data were processed with the computer PKIMLK program,including t-test.Results:The results indicate the levels of free radical and 6-Keto-PGF1ain diabetic rats are obviously higher than thoxein normal rats(P<0. 05).And the increase of free radicals is related to(6-Keto-PGF_(1α))/TXB_2(r=0.8796).Conclusions:The elevation of active oxygen free radicals in diabetic renal tissue seems to induce the increase of plasma 6-Keto-PGF_(1α),both cooperated with the change of early diahetic glomerular hemodynamies.