HPLC法检测血清胆红素辅助诊断Gilbert病

MEASUREMENT OF BILIRUBIN IN SERUM BY HPLC AND ASSISTANCE FOR DIAGNOSIS OF GILBERT'S SYNDROME

目的：了解Ｇｉｌｂｅｒｔ病发病情况，辅助临床诊断Ｇｉｌｂｅｒｔ病，分析不同类型黄疸和健康人的血清胆红素构成。方法：ＨＰＬＣ法。结果：“遗传性黄疸”组血清胆红素与文献报道的Ｇｉｌｂｅｒｔ病表现基本一致而与其它类型黄疸和健康人有明显差别。结论：临床上常规采用的重氮法，因过高估计血清结合胆红素而对Ｇｉｌｂｅｒｔ病几乎没有诊断价值，采用ＨＰＬＣ法分析血清胆红素构成，可将Ｇｉｌｂｅｒｔ病与正常人、溶血性黄疸、肝胆疾病鉴别开。

Objective:To understand clinical incidence and help diagnosis the Gilbert's syndrome,Meth-ods:HPLC.Results:the elevated serum bilirubin in“nclear reason”jaundice patients is mainly unconju-gated and have lower conjugated/total bilirubin;its serum bilirubin pattern is different from that of the healthy adult,hemolysis jaundice or hepatobiliary iaundice,but similar to the Gilbert's syndrome re-ported.Conclusions:there are many analysis methods for serum bilirubin,but there few methods are available in clinic for specific and accuracy determination of serum bilirubin fractions,so these methods are of no help for diagnosis of Gilbert's syndrome,which may be the one reason why few Gilbert's syn-dromes were reported in China;as the HPLC serum bilirubin determination method is more sensitive and specific,HPLC appears to be an excellent method to diagnose Gilbert's syndrome.