摘要
目的:制备抗tPA单克隆抗体,用于建立ELISA方法和研究tPA与肿瘤转移的关系。方法:用基因重组的组织型纤溶酶原激活剂(rtPA)免疫Balb/c小鼠,按常规法作细胞融合,用ELISA法筛选出阳性反应的杂交瘤细胞克隆。结果:获得一株杂交瘤细胞株(2B7);其免疫球蛋白亚类为IgG1;经ELISA法和免疫印迹技术证明,此单抗能特异性识别tPA。结论:用2B7McAb特异性强,用其建立的夹心ELISA检测tPA的方法线性较好,灵敏度可达1ng/ml。2B7McAb可望用于tPA与肿瘤转移关系及导向溶栓的研究。
Objective:In order to prepare anti-tPA monoclonal antibody,which is used to study the role of tPA in tumor metastasis.Methods:Babl/c was immunized peritoneally with rtPA and complete con-jugate.Then,immunized spleen cells and SP2/0 were fused in the help of PEG,Positive clones were selected with ELISA. Results:One hybridoma(2B7)secreting specific anti-tPA McAb was prepared.The McAb is subclassed into IgG1.Conclusions:2B7 McAb react specifically with both recombinant and natural tPA,but not with irrelevant proteins,ELISA kit established with 2B7 McAb showed high sen-sitivity and specificity.
出处
《北京医科大学学报》
CSCD
1996年第1期70-72,共3页
Journal of Peking University(Health Sciences)
关键词
单克隆抗体
肿瘤转移
纤维蛋白溶酶原
激活物
Headings Antibodies,monoclonal Neoplasm metastasis
Plasminogen activator,tissue-type