利用多聚赖氨酸包被球囊导管向血管特定节段转移基因

USING POLYLYSINE-COATED BALLOON CATHETER TO LOCALLY TRANSFER GENE INTO RABBIT ARTERIES

目的：研究多聚赖氨酸包被的球囊导管作在体定向基因转导的可行性。方法：将带有报告基因的腺病毒载体和空载腺病毒分别导入实验及对照组动物的特定动脉。结果：应用该系统，成功地将报告基因导入兔动脉壁中。结论：该局部基因转导系统可用于在体定向基因转导。

Objective . This study was undertaken to test the feasibility of local gene transfer in vivo us-ing polylysine- coated balloon catheter. Methods . Ad5 vector with reporter gene and empty Ad5 vector were locally transfered into experimental or control arteries. respectively.Results. with the local gene delivery system. reporter gene was successfuly transfeied into the artery wall.Concluslon. The polyly-sine- coated balloon catheter is feasible in in vivo local gene transfer.