摘要
目的：探讨蛋白激酶Ｃ（ＰＫＣ）在大鼠小肠缺血预处理中的作用。方法：采用大鼠小肠原位灌流的缺血再灌注（Ｉ／Ｒ）模型，观察ＰＫＣ抑制剂ＰｏｌｙｍｙｘｉｎＢ和Ｈ＿７对缺血预处理（ＰＣ）的肠道组织及血管床保护作用的影响。结果：ＰＣ明显减轻了Ｉ／Ｒ后肠粘膜病理损伤、ＭＤＡ产生、ＩＤＨ漏出（Ｐ＜０．０１），并减少了肠道微血管壁对伊文思兰的渗出（Ｐ＜０．０１）。ＰｏｌｙｍｙｘｉｎＨ和Ｈ＿７完全阻断了这种保护作用。结论：缺血预处理对Ｉ／Ｒ的小肠组织和血管床有保护作用，其机理涉及ＰＫＣ的激活。
Objective:To invextigate the effect of PKC on ischemic preconditioning(IPC) of rat intes-tine.Methods:On the model of in situ intestinal ischemia/reperfusion (I/R) injury of rat. the effeets of PolvmvxinBand H-(PKC inhbitors ) on intestinaltissue and vasculature protection of IPC were ob-served. Results:IPC signifleantly.attenuated intestinal mueosal lesionand leakage of LDH.CD.low-ered inerease of MDA content in intestinal tissue and vaseular permeability to Evans blue dve.These protective effeets were completly.abolished by administration of PKC inhibitor .Polymysin B or H_7. Conclusion:IPC Droteets intestinal tissue and vasculature from 1/R injury and these protective effeets are mediated by PKCaetivation.
出处
《北京医科大学学报》
CSCD
1996年第2期86-88,共3页
Journal of Peking University(Health Sciences)
关键词
再灌注损伤
蛋白激酶
小肠缺血
预处理
HeadingsIntestine,small /bloodsupply
Reperfusion injury/physiopathol
Protein kinaseC/ pharmacol
