枸杞多糖对小鼠胸腺细胞凋亡的调节

THE REGULATION OF LYCIUM BARBARUM POLYSACCHARIDE ON APOPTOSIS OF MOUSE THYMOCYTE IN VITRO

目的：以ＤＥＸ诱导小鼠胸腺细胞凋亡为模型，研究中药枸杞多糖（ＬｙｃｉｕｍＢａｒｂａｒｕｍＰｏｌｙｄｓｃ－ｃｈａｒｉｄｅ．ＬＢＰ）及氧化苦参碱（ＯＸＹ）对胸腺细胞凋亡的调节作用。方法：应用ＰＩ法检测亚２倍体细胞，二苯胺法测定胸腺细胞ＤＮＡ片断化％及ＤＮＡ凝胶电泳。结果：ＬＢＰ可抑制ＤＥＸ诱导的ＤＮＡ片断化，其抑制作用具有剂量依赖性，以１ｇ／Ｌ最明显；ＬＢＰ（ｌｇ／Ｌ）尚可阻止ＤＥＸ诱导的胸腺细胞内Ｃａ￣（２＋）升高。结论：ＬＢＰ可抑制ＤＥＸ诱导的小鼠胸腺细胞凋亡，ＯＸＹ对此无明显的调节作用。

Objective. The apoptosisofmurine thymocytes induced by DEX was regulated by LBP and OXY. Methods. PI staining. DNA fragmentation. DNA gel electrophoresis.and so on Results.LBP could inhibit fragmentation of DNA in murine thymocyte induced by DEX.The inhibition is dose-depen-dent and obvious. especially in 1 g/L.Ithas been shown that LBP could greatly reduce the rising of in tracellular Ca2+concentrathn induced by DEX or A23187 respectively.(%of inhibition .54 and 13).Conelusion. LBPcould inhibit the apoptosis induced by DEX.which OXY doesn’t have any effect on.