黄腐酸和超氧阴离子对软骨细胞内钙离子浓度的影响

THE EFFECT OF FULIC ACID AND SUPEROXID ANION ON INTERCELLULAR CALCIUM ION CONCENTRATION

目的：为探讨病区黄腐酸（ＦＡ）和活性氧自由基可能引起大骨节病，我们测定了在ＦＡ和超氧自由基（）作用下，软骨细胞内钙离子浓度（［Ｃａ￣（２＋）］ｉ）随时间的变化。方法：应用萤光指示剂Ｆｕｒａ－２／ＡＭ测定细胞内钙离子浓度并用下式计算：［Ｃａ￣（２＋）］ｉ＝Ｋｄ（Ｆ－Ｆ＿（ｍｉｎ））／（Ｆ＿（ｍａｘ）－Ｆ）。结果：作用４５ｍｉｎ后，［Ｃａ￣（２＋）］ｉ从１．６２×１Ｏ￣（－７）ｍｏｌ／Ｌ升达１．１８×１０￣（－６）ｍｏｌ／Ｌ；ＦＡ作用４ｈ后，［Ｃａ￣（２＋）］ｉ从３．７８×１０￣（－７）ｍｏｌ／Ｌ升达５．５１×１０￣（－７）ｍｏｌ／Ｌ。结论：说明ＦＡ与·有相似的促进［Ｃａ￣（２＋）］ｉ升高的作用，［Ｃａ￣（２＋）］ｉ的升高将造成软骨细胞损伤，而软骨坏死是大骨节病的重要病理特症之一。

s Objective . The variation of intercellular calcium ion concentrations of chondrocytes([Ca￣(2+)] )itreated by fulic acid(FA ) and superoxide anion()was measured.so that we can understand kashin-Beck’s Disease (KBD) induced by FA and oxygen free radical. Methods. [ Ca￣(2+)]iwas measured by using fluorescent indicator. Fura-2/AM and calculated with the formula[Ca￣(2+)]i=Kd (F-F_(min))/ (F_(max)-F).Results . These results indicated that [Ca￣(2+)]i increased from 1. 62 × 10￣(-7) mol/L to 1. 18 ×10￣(-6) mol/L when treated by· for 45 min and from 3. 78× 10￣(-7) mol/L to 5.51× 10￣(-7) mol/L when treated by FA for 4 h. Conclusious . Both FA and· made [Ca￣(2+)]iincrease. which will cause dam- age to chondrocytes. The cartilage necrosis is one of the pathological Characteristics of KBD.