一种高效而经济获得长片段基因突变体的方法

An Efficient and Economic Method of Obtaining Mutant Constructs of Large DNA Fragment

重组PCR方法的发展为体外突变技术提供了更快捷、准确的方法.通过改进引物设计和PCR保真性等方法,应用重叠延伸PCR的原理成功地实现了对BA基因的定点突变,并构建了其含有m1,m2,m3和m4的突变体.实验证明通过这种改进方法可以有效地进行长片段基因的突变,并且这种方法与其他方法相比具有简便、快捷、经济、高效的特点.

The development of recombinant PCR provides more rapid and correct method for mutation in vitro. Through improving primer design and PCR fidelity, site-directed mutagenesis and mutant constructs （ml, m2, m3 and m4） were obtained successfully, based on mechanism of overlap extension PCR. The results that this method can be used to obtain site-directed mutagenesis of large DNA fragment more conveniently, rapidly, economically and efficiently.