牛磺酸对原代培养新生大鼠乳鼠心肌细胞和心肌成纤维细胞的作用

Effect of taurine on primary cultured neonatal rat myocardial cells and cardiac fibroblasts

目的：探讨牛磺酸（Tau）对原代培养的新生大鼠乳鼠心肌细胞的保护作用及对心肌成纤维细胞的生长抑制作用。方法：将心肌细胞分为对照组、H2O2损伤模型组以及H2O2损伤加药物治疗组：阳性药川芎嗪（Lig）组及Tau大、中、小（80、40及20mmol·L^-1）剂量组，预先24h给予治疗药物后使用0．5mmol·L^-1 H2O2损伤心肌细胞6h，观察Tau对心肌细胞形态学的影响，MTT法测定反映心肌细胞存活力的A值以及测量乳酸脱氢酶（LDH）、超氧化物歧化酶（SOD）和丙二醛（MDA）含量的变化；将心肌成纤维细胞随机分为7组，分别给予0、10、20、40、80、160及320mmol·L^-1 Tau，MTT法测定不同浓度Tau对心肌成纤维细胞生长的影响。结果：H2O2损伤心肌细胞6h后，Tau（80、40及20mmol·L^-1）剂量组A值分别为0．479±0．055、0．437±0．052及0．421±0．062，与H202组（0．304±0．050）比较，差异均具有显著性（P〈0．001或P〈0．01）；Tau（80及40mmol·L^-1）剂量组使H2O2损伤的心肌细胞LDH释放减少、SOD含量增加、MDA含量降低，与H2O2损伤组比较差异均具有显著性（P〈0．01或P〈0．05）；40～320mmol·L^-1 Tau抑制心肌成纤维细胞的生长，与空白对照组比较差异具有显著性（P〈0．05，P〈0．01或P〈0．001）。结论：Tau可通过减轻自由基对心肌细胞的损伤，抑制心肌成纤维细胞生长，达到心脏保护作用。

Objective To investigate the protective effect of taurine on primary cultured neonatal rat myocardial cells and the inhibitory effect on cardiac fibroblast proliferation. Methods The myocardial cells were divided into six groups： control group, H2 O2 damage group, H2O2 damage group treated with ligustrazine and H2O2 damage group treated with taurine with different） doses （80, 40 and 20 mmol · L^-1）. Morpholoical changes of myocardial cells damaged for 6 h by 0. 5mmol · L^-1 H2O2 under the inverted microscope were observed. Meanwhile, cell survival ability and the contents of LDH, SOD and MDA were measured. Cardiac fibroblasts were randomly divided into 7 groups and co-cultured for 72 h with taurine with doses of 0, 10, 20, 40, 80, 160 and 320mmol · L^-1 and proliferation ability was assessed by MTT method. Results Absorption values in H2O2 damage groups treated with taurine （80, 40 and 20mmol · L^-1） were 0. 479±0. 055, 0. 437±0. 052 and 0. 421±0. 062, respectively. They were higer than that in H2O2 damage group （0. 304±0. 050） （P〈0. 001 or P〈0.01） ; LDH release and MDA content were remarkly decreased and SOD contents were increased in 80 and 40 mmol · L^-1 taurine groups, as compared with H2O2 damage group （P〈 0. 01 or P〈 0. 05）. Cardiac fibroblast proliferation was inhibited significantly in 40--320mmol · L^-1 taurine groups than that in control group （P〈0. 05, P〈0. 01 or P〈0. 001）. Conclusion Taurine can protect cultured neonatal rat myocardial cells from free radical damage and inhibit cardiac fibroblast proliferation.