吐尔基山辽代贵族墓葬人骨遗骸线粒体DNA多态性分析

Mitochondrial DNA analysis of Turki human remain

目的:利用分子生物学方法确定吐尔基山辽墓主人的身份及其与契丹贵族的关系。方法:对吐尔基山辽墓出土的人骨遗骸进行mtDNA研究,使用4对套叠引物对线粒体第一高可变区(360 bp)进行扩增和测序,利用契丹贵族人群的相关序列,对所得序列进行分子系统学分析。结果:与剑桥序列进行比对,共有5个突变位点;系统发育分析结果表明,吐尔基辽与契丹贵族人群遗传距离最近,且在契丹贵族的2个家族中与耶律羽之家族的亲缘关系相对较近,验证了吐尔基山辽墓主人的贵族身份。此外,与其他已报道人群数据进行比较表明,吐尔基山辽墓主人与现代外蒙古人群遗传距离最近,其5个突变位点均为蒙古人的突变热点。结论:吐尔基山辽墓样本应属于北亚蒙古人种。

Objective To identify the status of Turki human remain and its relation to Khitan nobles by the means of molecular biology. Methods MtDNA was successfully extracted from Turki human remain. Through four overlapping primers, the nucleotide sequence of 360 bp length was gotten. Then it was contrasted with the cambridge reference sequence （CRS）, and the sequence was analyzed with that of Khitan nobles basing the methods of phylogenetics. Results The result showed that Turki had closest relationship with Qidan noble individuals. And it was closer with Yeluyuzhi＇s family in the two Qidan noble families. It was proved that Turki was the noble of Khitan. Besides, it showed that Turki had closest relationship with modern out Mongolia contrasted with the reported populations. And the 5 mutation sites of Turki were all hot mutation sites of Mongolian. Conclusion Turki is north Mongolian.