不同载体蛋白制备的A群脑膜炎球菌多糖结合物的免疫原性比较

Immunities of Meningococcal Serogroup A Polysaccharide-Protein Conjugate Vaccines Prepared with Different Carrier Proteins

目的比较不同载体蛋白(TT、DT和rEPA)制备的A群脑膜炎球菌多糖(GAMP)结合物的免疫原性。方法采用溴化氰(CNBr)活化多糖,以无水己二酸二肼(ADH)为连接剂,1-乙基-1-3-(3-二甲基氨基-丙基)-碳化二亚胺(EDAC)为偶联剂,制备结合物并免疫小鼠,检测小鼠血清中抗GAMP与抗载体蛋白抗体及抗GAMP抗体的杀菌活性。结果多糖衍生物、多糖-蛋白质结合物均具有GAMP抗原特异性;结合物免疫小鼠可诱生较单独多糖更高水平的血清抗GAMP IgG抗体和更强的体外杀菌活性,并能产生免疫记忆。结论3种载体蛋白制备的结合物均能提高多糖抗原的免疫原性。

Objective To compare the immunities of meningococcal serogroup A polysaccharide （GAMP）-protein conjugate vaccine prepared with different carrier proteins （Tr, DT and rEPA）. Methods Activate CAMP with cyanogens bromide （CNBr） and prepare CAMP-protein conjugatcs using ADH as a linker and EDAC as a coupling agent. Immunize mice with the conjugates and determine the antibodies against CAMP and carrier proteins in their sera as well as the bactcricidal activity of antibody against CAMP. Results Both GAMP derivatives and GAMP-protein conjugates showed CAMP antigen specificity. Compared with CAMP alone, the GAMP-protein conjugates induced high serum anti-CAMP IgC antibody level and strong bactericidal activity in vitro. Thc conjugate also induced immunological memory. Conclusion All the CAMP-protein conjugates prepared with carriers proteins Tr, DT and rEPA enhanced the immunogenicity of GAMP.