蛋白对HCV/HBV DNA疫苗的免疫增强作用

Immunogenicity of Combined HCV/HBV Immunization by DNA Prime-Protein Boost Strategy

目的探讨蛋白对HCV/HBV DNA疫苗的免疫增强作用。方法将构建的HCV/HBV真核表达载体pcDNA-PCXS和蛋白分别免疫BALB/c小鼠,用ELISA的方法检测抗-HBs和抗-HCV Ab;3H-TdR掺入法检测免疫小鼠T淋巴细胞增殖;51Cr释放法检测免疫小鼠特异性CTLs杀伤作用。结果DNA-蛋白组抗-HBs和抗-HCV抗体均较DNA组出现早,且抗体水平明显高,DNA-蛋白组的CTL应答水平也明显高于DNA组。结论蛋白能提高DNA疫苗的特异性体液和细胞免疫功能,为DNA疫苗的实际应用提供了一种新的策略。

Objective To explore the effect of hepatitis C virus（HCV）/hepatitis B virus（HBV） bivalent vaccine by a primeboost strategy. Methods Immunize BALB/c mice with the constructed eukaryotic expression vectors PeDNA-PCXS,pcDNA-PCSS ＋ S/ PCX protein and pcDNA3, 0 respectively. Determine anti-HBs and anti-HCV antibodies by ELISA,T lymphocyte proliferation by ^3HTdR incorporation and specific killing activity of CTLs by ^51Cr release assay. Results Compared with those immunized with pcDNAPCXS,high anti-HBs and anti-HCV titers were induced early in mice immunized with pcDNA-PCXS ＋ S/PCX protein. The specific CTL response level induced by pcDNA-PCXS ＋ S/PCX protein was significantly higher than that induced by pcDNA-PCXS. Conclusion DNA prime-protein boost immunization enhanced the humoral and cellular immunological functions of immunized mice. It provided a novel strategy for the practical application of DNA vaccine.