栝楼核糖核酸酶在RNA序列分析中的应用

Application of RNase TCS in RNA Sequence Analysis

栝楼核糖核酸酶（ＲＮａｓｅＴＣＳ）对Ｕ碱基具有高度的专一性，在无脲、ｐＨ３．５、５０℃时，它几乎都在－Ｎｐ↓Ｕ－处裂解ＲＮＡ它与ＲＮａｓｅＴ１，Ｕ２和有限的碱水解一起，可用于直接的酶法ＲＮＡ序列分析．

RNase TCS isolated from plant Trichosanthes Kirilowii Maxim has a highuracil-specific activity. In the absence of urea and under conditions of PH3. 5, 50℃, it cleaves almost exclusively and uniformly at -NP ↓ U-. Inconjunction with RNase T1, U2 and limited alkaline hydrolysis, the RNase TCS is useful in direct enzymatic RNA sequence analysis.