McAbGB_2识别的乳腺癌血清抗原性质研究

A Study on Character of Serum Antigen Recognized by McAbGB_2 in Breast Cancer

对抗人乳腺癌血清抗原单克隆抗体ＧＢ２（ＭｃＡｂＧＢ２）识别的抗原（ＭｃＡｂＧＢ２抗原）性质及含量进行了研究．结果表明，ＭｃＡｂＧＢ２抗原是由糖及蛋白质组成的复合蛋白质，不耐热；ＭｃＡｂＧＢ２抗原决定簇不存在于铁蛋白及癌胚抗原；蛋白质印边检测表明ＭｃＡｂＧＢ２抗原有两条区带，分子量分别为１１６及４５ｋｕ，分布于血液及癌组织；用ＭｃＡｂＧＢ２进行血清学分析（ＥＬＩＳＡ试验），结果表明：乳腺癌阳性符合率达９８％（５０／５１），正常人及乳腺良性肿瘤病人的假阳性率分别为６％（３／５０）及６．７％（１／１５）．ＭｃＡｂＧＢ２抗原可能是新的乳腺癌相关抗原．

A study on character and content of antigen recognized by McAbGB2 against serum antigen of human breast cancer was reported.The results demonstrated that the antigen recognized by McAbGB2 is a complex protein which is composed of carbohydrate and protein and it is susceptible to heat. Competitive ELISA test showed that the binding of McAbGB2 to its antigen could not be inhibited by ferritin and CEA.Western blot revealed that McAbGB2 recognized antigen with molecular weight of 116 and 45 ku.The antigen recognized by McAbGB2 exists in breast cancer tissue and serum. ELISA test with McAbGB2 showed the rate of accord with positivity is 98% (50/51 ) in breast cancer patient sera and the rates of false positivity are 6%(3/50) in normal serum samples and 6.7%(1/15) in benign breast disease sera respectively. The antigen recognized by McAbGB2 may be a new tumor-associated antigen.