铽离子（Ⅲ）和5－FSAP在TrF中增强效应研究

Laboratory Research in Enhance Effect of Tb(Ⅲ)and 5-FSAP on TrF

为利用灵敏的酶增强时间分辨荧光免疫分析（ＥＡＴｒＦＩＡ）测定生物活性物质，对三价铽离子［Ｔｂ（Ⅲ）］，５－氟水杨酸磷酸盐（５－ＦＳＡＰ）和碱性磷酸酶（ＡＰ）的实验条件进行了探讨，并发现它们之间有着一些定量的关系．这些研究结果为时间分辨荧光分析（ＴｒＦ）的发展积累了有益的资料．

To study the ultrasensitive enzymatically amplified time-resolved fluorescenceassay (TrF ) of bioactive matter, the experimental condition of trivalent terbium ion [Tb (Ⅲ) ], 5-fluorosalicyl phosphate ester (5-FSAP) and alkaline phosphatase (AP) have been investigated. It is found that there are some quantitative relationships between them. These results supply some good information for development of TrF.