转基因猪体内外源基因的整合鉴定

Identification of Integration of Foreign Gene in Transgenic Pigs

外源猪生长激素基因被导入湖北白猪．为避免羊、猪之间在金属硫蛋白启动上的同源性而造成ＰＣＲ检测结果的假阳性，分别将ＰＣＲ合成反应的两个引物设计在羊金属硫蛋白基因启动子一侧和猪生长激素一侧，使ＰＣＲ合成反应的结果特异地反映外源基因的整合性，准确地鉴定出转基因猪．ＤＮＡ印迹分析结果表明，４３头待检仔猪中有６头呈外源基因整合阳性．

Foreign porcine growth hormone genes were introduced into Hubei white pigs. Inorder to avoid possible homology in metallothionein-Ia gene promoter between sheep and porcine, two PCR primers were designed and located on the regions of sheep metallothionein Ia gene promoter and porcine growth hormone gene, respectively. This reflects the exact results by PCR detection. The results of DNA blotting indicated that 6 of 43 offsprings showed positive integration.