耗竭性运动对大鼠骨骼肌线粒体内膜的影响

Influence of Exhaustive Exercise on Innermitochondrial Membrane of Skeletal Muscle in Rats

观察ＳＤ大鼠一次急性运动至力竭后骨骼肌线粒体内膜流动性、ＮＡＤＨ－ＣｏＱ还原酶及ＡＴＰ酶活性变化．结果显示，大鼠骨骼肌线粒体内膜微粘度较安静时显著增高，线粒体内膜ＮＡＤＨ－ＣｏＱ还原酶和ＡＴＰ酶活性分别较安静时下降３４．２％和４６．２％．研究提示，耗竭性运动后大鼠骨骼肌线粒体呼吸链内膜分子动力学和呼吸链酶组分活性变化，可能是运动性疲劳重要的膜分子特征．

Rats running with an incrementalintensity to exhaustion were used as an experimental model to investigate the effect of acute exhaustive exercise on inner-mitochondrial membrane of skeletal muscler. It was found that, in exhausted rats, fluorescence polarization and membrane microviscosity of inner mitochondrial membrane increased significantly and the activities of NADH-CoQ reductase and ATPase in respiratory chain declined by 34 .2% and 46 .2 %,respectively. The result suggested that the functional alterations in mitochondrial membranes such as the changes on membrane dynamic and activities of respiratory chain enzyme may be one of membrane molecular characteristics for exercise induced fatigue.