快速检查寡聚DNA片段序列的方法

A Method to Confirm the Sequence of a Defined DNA Primer

以质粒为模板，用待测寡聚ＤＮＡ片段和通用测序引物进行ＰＣＲ（聚合酶链式反应），ＰＣＲ片段经纯化后插入到ｐＵＣ－１８或ｐＵＣ－１９的多克隆位点中，然后用通用测序引物测定重组质粒上待测寡聚ＤＮＡ片段，即可清晰、正确地知道它的序列．

The sequence of a defined DNA primer can be detected easily by a method described as below. A PCR (polymerase chain reaction) was carried out with the primer to be detected and one of the M13 universal sequencing primers. The resulting PCR fragment was purified and inserted into a PUC-18 or pUC-19 at the multicloning sites. Then the sequence of the primer to be detected can be exactly known by sequencing the recombinated plasmid with a M13 universal sequencing primer.