农杆菌LBA4404不含内源GUS基因

Agrobacterium tumefaciens LBA4404 Lacks Intrinsic β-Glucumidase(GUS)Gene

用长度为２１ｂｐ的一对β－葡糖苷酸酶（ＧＵＳ）基因的ＰＣＲ引物，从根癌农杆菌ＬＢＡ４４０４细胞总ＤＮＡ中扩增不出任何片段，但从合ｐＢＩ１２１的ＬＢＡ４４０４细胞总ＤＮＡ中可扩增出一条预期大小（１．２ｋｂ）的ＧＵＳ基因片段，这表明根瘤农杆菌ＬＢＡ４４０４不含内源ＧＵＳ基因．

Direct evidence for the lack of intrinsic β-glucuronidase (GUS) gene in Agrobacterium tumefaciens LBA4404 was presented using PCR technique. No amplified fragment was obtained with a 21 bp primer pair, which is specific for the sequence of the GUS gene, from total cell DNA of the Agrobacterium tumefaciens LBA4404, whereas a single 1 .2 kb fragment, which is identical in size to that predicted for the internal GUS gene fragment,was amplified with the same primer pair from total cell DNA of the LBA4404 containing the plasmid pBI121 and from purified PBI121 respectively.