AMACER CK34βE12在前列腺穿刺活检组织中的表达

The Expression of AMACER and CK34βE12 Immunohistochemistry in Prostatic Puncture Biopsy Tissue

目的:探讨AMACER和CK34βE12在前列腺穿刺活检组织中的表达,评估其在前列腺癌诊断上的意义。方法:应用免疫组织化学法检测178例前列腺穿刺活检组织中AMACER和CK34βE12表达情况,其中前列腺腺癌(Cap)69例、良性前列腺增生(BPH)101例、前列腺上皮内组织瘤(PIN)5例、不典型小腺体增生(ASAP)3例,结合临床资料进行分析。结果:AMACER在微粒穿刺活检组织中CaP阳性表达率为100%,其中弥漫阳性率(≥+++)为98.6%;BPH中(-)84例(83.2%),(+)17例(15.8%),(++)1例(1.0%);PIN和ASAP均为阴性表达。CK34βE12在全部CaP和ASAP病例中为阴性表达,在BPH和PIN病例中均为阳性表达.结论:AMACER是前列腺腺癌的特异性肿瘤标记物,将其与CK34βE12联合应用于穿刺活检所获得前列腺微粒组织的检测,可以确诊前列腺癌,提高早期癌和小体积癌(<0.5ml)的检出率。

Objective： To investigate the expression of AMACER and CK3413E12 in small-volume prostatic biopsy tissue and to evaluate their significance in detection of prostatic adenocarcinoma （Cap）. Methods： The microamount punctio specimens of 178 prostatic cases, including Cap （n=69）, benign prostatic hyperplasia （BPH, n=101）, proststatic intraepithelial neoplasia （PIN, n=5） and atypical small acinar proliferation （ASAP, n=3）, were stained by immunohistochemical analysis of AMACER and CK3413E12. Results： In the microamount punctio specimens of 178 prostatic cases, AMACER was strongly and diffusely positive（≥＋＋＋） in 98.6% of total 69 Cap cases and focally positive（＋）in 1 case. In 101 cases of BPH, 17 （15.8%） showed （＋） staining and 1 case （1.0%） showed （＋＋） staining. AMACER was negative in all 5 cases of PIN and 3 cases of ASAP. The CK3413E12 was negative in Cap and ASAP cases, it was strongly positive in BPH and PIN cases. Conclusions： AMACER is a kind of specific positive tumor marker for prostatic adenocarcinoma. AMACER combined with CK3413E12 in puncture biopsy of prostate can improve the detection rate of early prostatic adenocarcinoma and the tumors with less than 0.5 ml.