摘要
作者从拘橼酸杆菌中提取纯化获得其Ｖｉ多糖抗原，该抗原具有伤寒沙门氏菌Ｖｉ抗原的免疫学特性，而不含伤寒沙门氏菌０，Ｈ抗原，用其致敏新鲜羊血球作被动血凝试验，特异性敏感性均很好。所需Ｖｉ抗原致敏浓度极低，仅为０．０５ｕｇ／ｍｌ。使用新鲜羊血球凝模式较好，便于观察结果，采用被动血凝试验检测１０６名健康中学生肌肉注射３０ｕｇ伤寒Ｖｉ多糖菌苗前后Ｖｉ抗体的变化情况，发现免疫后血清抗体的四倍增长率达８９％，表明伤寒Ｖｉ多糖苗具有良好的免疫原性。
The authou isolated and puified the Vi polysaccheride from Citrobacter. This polysaccharide posses sed the immunity of Salmonella typhi Vi polysaccharide,but didn't contain the O,H antigen of S.typhi. Using the purified Citrobacter Vi for Passive hemagglution, it got good results both in specility and sensitive. The Vi working-concentration was very small,only 0.05μg/ml. Using the fresh sheep blood cell,the agglution pattern was fine. With this method,the author decteced 106 students'serologic response to typhoid Vi polysaccharide veccine,seroconversions was 89%. this result implicated that the typhoid Vi capsular polysaccharide vaccine we used had good immunogenicity.
出处
《微生物学免疫学进展》
1996年第1期28-31,共4页
Progress In Microbiology and Immunology
