鼻咽癌组织中转移抑制基因和血管内皮生长因子蛋白表达及其临床意义

Expression clinical significance of nm23-H_1 and vessel endothelium growth factor protein in nasopharyngeal carcinoma

目的探讨转移抑制基因(non-metastatic gene-23-H1,nm23-H1)和血管内皮生长因子(vessel endothelium growth factor,VEGF)蛋白表达与鼻咽癌(nasopharyngeal carcinoma,NPC)预后的相关性。方法对108例鼻咽癌放疗前活检标本进行检测,应用免疫组织化学技术检测nm23-H1和VEGF基因蛋白在鼻咽癌组织中的表达情况,并分析两基因的表达与鼻咽癌分期、放疗敏感性、生存率、转移及复发之间的关系。结果鼻咽癌组织中nm23-H1和VEGF蛋白阳性表达率分别为48·1%和59·3%。单因素分析表明,鼻咽癌分期、淋巴转移、生存率及远处转移与nm23-H1蛋白低表达均有密切关系,而VEGF的高表达则与鼻咽癌的分期、淋巴转移、放疗敏感性、生存率及复发均有密切关系。nm23-H1蛋白表达与VEGF表达呈负相关(r=-0·577,P<0·05)。结论nm23-H1蛋白低表达和VEGF高表达对判断病变进展,预测放疗敏感性,生存率及转移和复发有重要参考价值。

Objective To study the prognostic predictor for nasopharyngeal carcinoma （NPC）. Methods The expressions of nm23-H1 and vessel endothelium growth factor （VEGF） protein were examined by immunohistochemistory S-P staining in 108 NPC tissues, the expression of nm23-H1 and VEGF protein in NPC tissues with clinical stage of NPC, radiosensitivity of tumor, survival rate of patients, relapse and metastasis of carcinoma were studied. Results The positive rate of nm23-H1 and VEGF was 48. 1% and 59. 3% respectively. The clinical staging, metastatic potential of lymph nodes were correlated with low-level expression of nm23-H1 protein. The patients with negative nm23-H1 expression had worse prognosis than those with positive nm23-H1 expression. The clinical staging, metastatic potential and poor sensitivity of radiotherapy were correlated with high level expression of VEGF protein. The patients with positive VEGF expressin had worse prognosis than those with negnative VEGF expression. The expression of nm23-H1 protein was negatively correlated with the expression of VEGF protein （ r = - 0. 577, P 〈 0. 05 ） . Conclusions The low level expression of nm23-H1 protein and the high level expression of VEGF protein may be associated with the development and poor prognosis of NPC.