对小鼠黑色素瘤细胞肺转移的抑制作用mGM-CSF基因转染

Inhibition of lung metastasis of B16 melanoma cells transfected with murine granulocyte-macrophage colony-stimulating factor in mice

目的探讨小鼠粒细胞巨噬细胞-集落刺激因子(mGM-CSF)基因转导对B16黑色素瘤细胞肺转移的抑制效果.方法应用脂质体将含有mGM-CSF基因的真核表达载体转染B16小鼠黑色素瘤细胞,经G418加压筛选后,挑选表达mGM-CSF基因的B16黑色素瘤细胞.观察转导或未转导mGM-CSF基因的B16黑色素瘤细胞在Balb/c小鼠体内的肺转移.结果转导mGM-CSF基因的B16黑色素瘤细胞可以稳定表达mGM-CSF,在小鼠模型上其肺转移灶数目显著少于未转染基因的黑色素瘤细胞对照组,二者差异显著(n=5,125.8±34.3vs27.8±24.0,P<0.01).结论mGM-CSF转导可显著抑制小鼠黑色素瘤细胞B16的肺转移,提示GM-CSF具有治疗黑色素瘤的临床应用前景.

AIM： To investigate the inhibition of lung metastasis of melanoma by a tumor vaccine of B16 melanoma cells transfected with murine granulocyte-macrophage colony-stimulating factor （mGM-CSF）. METHODS： The murine melanoma cell line B16 was transfected with the recombinant plasmid containing mGM- CSF. A cell line stably expressing mGM-CSF was obtained by G418 selection. The Balb/c mice were administered i. v. with B16 cells transfected with or without mGM-CSF respectively and the lung metastasis was examined 2 weeks later. RESULTS： The recombinant cell line B16-TR was established after mGM-CSF tranfection and the expression of mGM-CSF was confirmed by RT- PCR. The number of lung metastatic lesions of mice treated with B16-TR was significantly fewer than that of mice treated with B16 （n=5, 125. 8 ± 34. 3 vs 27. 8 ±24. 0, P 〈0. 01）. CONCLUSION： The mGM-CSF transfection can significantly suppress the lung metastasis of melanoma in mice. The GM-CSF can be a promising adjuvant in the immunotherapy of melanoma.