交流电电击小鼠眼损伤的电镜实验观察

Experimental Studies on Ocular Tissues Taken from Mice of Electrocution under the Electron Microscope

目的:采用透射电镜观察电击死后小鼠眼损伤的超微形态学改变特点。方法:以9例实验小白鼠电击致死(220V,50Hz电流正极接头部,负极接右下肢),取眼球经戊二醛固定后,双眼按中轴线切开一分为二,取其中一半进行电子显微镜观察。结果:9例电击组小白鼠电击死后,眼组织中角膜、晶体、视网膜等均发生了不同程度的电镜形态学改变;5例非电击实验组小白鼠形态学改变不明显。结论:电击实验组小白鼠的眼睛组织在电镜下观察具有一定形态学改变特点,该改变特点对治疗眼部电击损伤有着一定的参考价值。

Purpose：To observe morphological characteristics in ocular tissues with electrocution under the electron microscope. Methods：Four-teen mice are divided into two groups, the experimental group and the control group. In the experimental group, all of the 9 mice are killed by electricity （220v, 50Hz）, with the heads connected to anode and the right leg to cathode. In the control group, all of the 5 mice are beheaded. Eyes fixed in glutaraldehyde, are split into two halves. One is observed under the electron microscope, and the other is observed morphological characteristics by H-E stain, which has been published. Results：In the experimental group, morphological characteristics of the components of eyes present in varied degrees under the electron microscope, but not obvious in control group. Conclusion：It can be drawn that these certain morphological characteristics in the experimental group might be helpful to the treatment of electrical injury.