灭活肿瘤细胞免疫小鼠诱生的自身抗体及其作用探讨

Study on the effect of anti-dsDNA autoantibody induced by inactivated tumor cell

目的探讨灭活肿瘤细胞免疫诱导机体产生抗dsDNA自身抗体及该自身抗体与抗肿瘤效应的关联性。方法以灭活SP2/0肿瘤细胞3次免疫Balb/c小鼠,末次免疫2周后采血,ELISA法检测血清中抗dsDNA自身抗体的表达水平。进一步用自身或不同来源的肿瘤细胞攻击经灭活SP2/0免疫的Balb/c小鼠,观察免疫保护效应;或以免疫血清与肿瘤细胞共孵育后接种小鼠,观察肿瘤生长和生存。结果灭活肿瘤细胞免疫小鼠可诱导产生较高滴度的抗dsDNA自身抗体。体内攻击实验表明:抗dsDNA自身抗体滴度的降低与肿瘤细胞攻击后小鼠肿瘤生长呈负相关,免疫血清与肿瘤细胞孵育后接种小鼠可明显延缓小鼠的成瘤时间,延长生存期。结论灭活肿瘤细胞免疫小鼠诱生的抗dsDNA自身抗体与体内抗肿瘤效应具有关联性。

Purpose To investigate whether anti-dsDNA autoantibody could be induced by inactivated tumor cell inoculation into Balb/c mice and the relationship between anti-dsDNA antibody and antitumor effect. Methods The anti-dsDNA autoantibody was detected via ELISA from the sera of Balb/c mice immunized with inactivated SP2/0 tumor cells, And the immune protection was observed by challenging SP2/0 or Renca tumor cell lines into SP2/0 immunized mice. Tumor growth and size were further measured by injection of tumor cells pretreated with the sera from immunized mice. Results A higher level of anti-dsDNA antibody could be detected by inactivated SP2/0 injection. Anti-dsDNA derived from SP2/0 tumor immunized mice could protect either self SP2/0 or nonself Renca tumor cell challenge. The retarded tumor growth and prolonged survival could be observed in mice inoculated with tumor cells pretreated with sera from immunized mice. Conclusions These results indicate that anti-dsDNA has closely associated with the anti-tumor effect .