摘要
目的观察人头皮毛乳头细胞海藻酸钠多聚赖氨酸海藻酸钠(alginatepolylysinealginate,APA)微囊是否具备诱导小鼠耳部毛囊再生的功能;寻找理想的微囊直径。方法以APA微囊包裹体外分离培养的人头皮毛乳头细胞;将毛乳头细胞微囊移植至小鼠耳部皮下,6周后局部取材行组织学检查;共聚焦显微镜下观察葡聚糖荧光素在APA微囊中的扩散速度和扩散方式,并对比相同时间、不同直径的APA微囊中葡聚糖荧光素的强度,综合分析确定最佳的微囊直径。结果组织学检查显示:移植部位皮下有密集的同心圆状毛囊结构形成,其数量、大小、分化程度等与对照组明显不同。荧光素以同心圆状、逐层渗透的方式向APA微囊中扩散;相同时间内荧光强度比较:小囊组>中囊组>大囊组。结论微囊化毛乳头细胞具备诱导毛囊再生的生理功能;微囊理想的直径是400μm。
Objective To induce the hair follicle regeneration in mice ear by microencapsulated dermal papillae cells(DPs) and to investigate the permeability of fluorescein in APA microcapsulation to search the ideal diameter of microcapsulation. Methods The DPs were encapsulated with alginate-polylysine-alginate by a highvoltage electric field droplet generator. The microencapsulated dermal papilla cells were xenotransplanted into the mice ears. After 6 week, the histological examination was made by microscopy. The diffusion way and speed of fluorescein into the microcapsulations were observed by confocal laser scanning microscopy. The comparison of fluorescein intensity was made in APA microcapsulations with different diameters. Results Fully developed hair follicles could be easily identified in the skin of implanted site following xenotransplantation of microencapsulation DPs, which were different from the control groups in configuration, number, size and differentiation degree. The fluorescein was diffused gradually into the microcapsulations with a shape of concentric circularity. The fluorescein intensity inside three groups of APA microencapsulations was : small 〉 middle 〉 big. Conclusions The microencapsulated DPs retain the physiological function to induce the follicle regeneration. The APA microcapsulations with 400urn diameter could ensure the nutrition and metabolite to pass in and out freely, and isolate the immunocompetent substance absolutely.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2006年第2期88-91,共4页
Chinese Journal of Plastic Surgery
基金
国家自然科学基金资助项目(30170973)
广东省自然科学基金资助项目(010435)
关键词
毛乳头细胞
微囊
毛囊再生
Dermal papilla cell
Microencapsulation
Hair follicle regeneration