摘要
目的探讨瘢痕疙瘩(K)上皮细胞生物学行为对毛囊皮脂腺(HFSG)转归的影响。方法收集K边缘部位和正常皮肤(NS)标本,采用组织化学和免疫组化染色方法,观察结构正常和异常的HFSG上皮细胞表达免疫黏附分子和细胞角蛋白14(CK14)以及局部活性淋巴细胞浸润情况。结果与NS不同,KHFSG上皮细胞异常增殖,结构畸变、解体或形成复层鳞状上皮(皮岛样)结构,其密度随着结缔组织增生、成熟而逐渐降低;在强表达细胞间黏附分子(ICAM)1、D相关人类白细胞抗原位点(HLADR)和CK14的KHFSG周围,有相应的CD4、CD45RO和γ干扰素(IFNγ)阳性免疫细胞浸润,二者之间统计学呈明显的正相关。结论KHFSG结构破坏与反应性上皮细胞增生免疫诱导增强有关。
Objective To investigate the influence of biological behavior of epithelial cells on the hair follicles and sebaceous glands (HFSG) structure in keloids (K). Methods The expression of intercellular adhesion molecule ( ICAM )-1, D-related human leucocyte antigen ( HLA-DR ), and cytokeratin (CK) 14 on epithelial cells and the amount of activity T-lymphocytes were detected in specimens of keloid edge and normal skin with immunohistochemical and histological methods. Results In comparison with normal skin specimens, epithelial cells were proliferated in K-HFSG presented structural aberration and disintegrate or abnormally to form solid-epithelial island-like structure, and the density of HSFG with hyperplasia and the ageing scar in keloids was apparently decreased . They strongly expressed ICAM-1, HLA-DR, and CK14 in the epithelial cells, there were many immunologic cells which expressed CD4, CD45RO, and interferen(IFN)-γ around the K-HFSG. The expressed level of epithelial cells was positively correlated with the density of immunologic cells nearby K-HFSG. Conclusion It could be concluded that the reactivity with hyperplasia and immunoinduction of epithelial cells might be associated with the destruction of the some HFSG structure in the keloids.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2006年第2期106-108,共3页
Chinese Journal of Plastic Surgery
基金
国家重大基础研究规划资助项目(G1999054204)
国家自然科学基金项目(30170966)
泰州市海陵区科委科研专项基金资助