摘要
基因工程人β干扰素工程菌经发酵培养、纯化后获得纯的制品，采用斑点杂交技术，用非放射性地高辛标记超声裂解的全工程菌ＤＮＡ作为探针，检测基因工程人β干扰素纯品，结果显示：基因工程人β干扰素纯品中的外源ＤＮＡ含量小于１００ｐｇ／剂。
rhIFN-β was expressed in E.coli strain and subseqent purified,the purity of final product was over 95%. Residual DNA in purified rhIFN-β was detected by means of dot blot hydridization,using total DNA of E.coli pBV 320 cells labelled with nonradioactive DIG as a probe,the result showed that residual DNA in final product was far less than 100 pg/dose.
出处
《微生物学免疫学进展》
1996年第2期46-48,共3页
Progress In Microbiology and Immunology
