痘苗病毒载体研究进展

Progress on the Study of Vaccinia Virus Vectors

制备高活性的真核蛋白，必须使用哺乳动物细胞，痘苗病毒为这一工作提供了简便、实用的工具．痘菌病毒宿主范围广，无致癌性，插入长达２０多ｋｂ的外源基因后仍有感染性，能够高效表达外源蛋白，有效地加工表达产物．而且，痘菌病毒的表达产物可刺激机体免疫系统产生良好的体液免疫和细胞免疫．

It is prospective to use mammalian cells for producing fully active eukaryotic proteins. Vaccinia virus vector provides a simple, practical means for this purpose. As a vector, vaccinia virus has a number of useful characters: wide host range, non-carcinogenicity,permitting of cloning large fragments of foreign DNA (>20 kb), retention of infectivity after insertion of foreign DNA, relatively high level of protein synthesis as well as 'appropriate' transportation, secretion, processing and posttranslation modifications. In addition, the expressed proteins can prime immune system well to develope humoral and cellular immunity. Vaccinevirus vector with high level expression will be or very useful in producing bioactive substances and constructing recombinant vaccines in mammalian cells.