运用差异展示分离特异性表达的基因

Isolate Specifically Expressed Genes by Differential Display.

在高等生物中含有约１０００００个不同的基因，其中仅有１５％的基因在任何个体细胞中均表达．因此分离特异的目的基因便显得十分重要差异展示是通过部分扩增ｍＲＮＡ的逆转录产物、经测序胶电泳，分离到差异性表达的基因它与消减杂交相比是分离特异表达基因的更有效的手段．虽然这种方法在实际运用中存在着这样或那样的困难，但随着对这种技术的不断改进，它将会有越来越广泛的用途．

There are about 100 000 different genes in higher organisms, and only 15% of them are expressed in any individual cell. Thus it is very important to isolate specific genes.Comparing with substractive hybridization, differential display is a more effective approach to isolate specifically expressed genes. In differential display, reverse transcripted products of mRNA are amplified and displayed on sequencing gel. Then differentially expressed genes are isolated. Although there are many difficulties in using this method, as further improved, it will be useful in various fields.