黄芪总黄酮对DNA损伤防护作用的研究

Protective Effect of Total Flavonoids Astragalus on DNA Strand Breaks in V79 Cells Induced by Hydrogen Peroxide and Gamma Rays.

用ＤＮＡ解旋荧光检测法（ＦＡＤＵ）研究了黄芪总黄酮（ＴＦＡ）对γ射线和Ｖ７９所致Ｖ７９细胞ＤＮＡ链断裂的防护作用结果表明ＴＦＡ对这两种损伤因子所致的ＤＮＡ损伤均有不同程度的防护作用，当ＴＦＡ浓度达到０．４ｇ／Ｌ和０．６ｇ／Ｌ时，分别对Ｈ２Ｏ２和γ射线所致的损伤有保护作用（Ｐ＜０．０５），而浓度增至０．８ｇ／Ｌ和１．２ｇ／Ｌ时，分别对两种因素所致的ＤＮＡ链断裂损伤有非常显著的防护效果（Ｐ＜０．０１），对Ｈ２Ｏ２的防护效果优于对γ射线．

The protective effect of total flavonoids Astragalus (TFA), one of the natural anti-oxide agent isolated from Astragalus monghlious, on DNA strand breaks induced by the hydrogen peroxide and gamma rays has been investigated with the fluorometry assay of DNA unwinding (FADU). The results showed that the TFA has different degree of protective effect on DNA strand breaks caused by hydrogen peroxide and gamma rays respectively. It also showed some protective effect on DNA strand breaks induced by these two damage factors,when the concentration of TFA were 0.4 g/Land 0. 6 g/L, respectively. When its concentrations were increased to 0. 8 g/L and I .2 g/L,TFA could effectively protect DNA from hydrogen peroxide and gamma rays damages. Compared the protective effect of TFA on DNA strand breaks induced by hydrogen peroxide and gamma rays, the former was better. The protective mechanism of TFA on DNA strand break could be related to savenging free radicals.