胚胎骨Ⅰ型胶原的提取与鉴定

Extraction and Identification of Type I Collagen from Human Embryonal Bone.

应用酸性、中性交互提取法从人胚骨中提取Ⅰ型胶原，经ＳＤＳ－ＰＡＧＥ电泳，氨基酸分析和免疫学方法鉴定．结果表明所提胶原电泳区带与Ⅰ型标准品相同，环状沉淀反应阳性，氨基酸分析甘氨酸占１０００个氨基酸总残基的１／３，羟脯氨酸与脯氨酸之比为０．６５，符合Ⅰ型胶原特征，并显示有较高的纯度，可用于胶原制品的制作．

Type I collagen was extracted from human embryonal bone by alternating acidic and neutral salt precipitation technique and was identified by SDS-PAGE, amino acid analysis and immunological method. The results showed that electrophoresis band of collagen extracted was the same as that of type Ⅰcollagen standard,ring precipitation test was positive, glycine was 1/3 in total of 1000 amino acid residues, the ratio of hydroxyproline and proline was 0.65.These suggested that the extracted collagen accord with the characteristics of type Ⅰcollagen and is highly purified. It could be used to prepare collagen products.