用漂片法研究中枢和周围神经内GABA能神经元和终末的免疫组织化学改良方法

AN IMPROVED IMMUNOHISTOCHEMICAL METHOD TO STUDY GABA-ERGIC NEURONS AND TERMINALS IN CENTRAL AND PERIPHERY NERVOUS SYSTEM WITH FREE FLOATING SECTIONS

ＧＡＢＡ的免疫组织化学定位研究是比较特殊且困难的方法，以往的研究在方法上存在许多缺憾。我们对此进行了改良：用０．５％戊二醛，４％多聚甲醛，０．２％苦味酸固定液灌注固定，振动切片，漂片方法，研究了中枢和周围神经系统中ＧＡＢＡ的免疫组织化学定位，得到了满意的结果，避免了内源性、代谢性ＧＡＢＡ呈色，达到快速、经济之目的。

It was more difficulty to localize GABA-ergic neurons and terminals immunohistochemically. There are many disadvent ages in previous method. An improved method, perfusing animals with a fixative containing 0. 5% glutaraldehyde, 4%paraformaldehyde and 0. 2% picric acid in 0. 1 mol/L phosphate buffer, free floating sections, is used to localize GABA-irneurons and terminals in the central and periphery nervous system. The method avoided staining of metabolic GABA in neurons and realized the aim of short span and economic.