摘要
将来自北京地区甘蓝和芥菜上的TuMV外壳蛋白基因片段分别克隆到pMD18-T载体中，测序并进行序列分析。结果表明：两者CP基因序列长度均为867个核苷酸，编码288个氨基酸，它们的核苷酸同源性为97．2％，氨基酸同源性为97．6％；两者与GenBank中的部分CP基因序列的核苷酸同源性在87．8％～97．8％之间，氨基酸同源性在91．7％～99．0％之间；分子进化树分析两者可归属World-B组。
Two samples of turnip mosaic virus,which infected cabbage and turnip in Beijing,were named TuMV - BJ1 and TuMV - BJ2, respectively. The CP genes of TuMV - BJ1 and TuMV - BJ2 were sequenced after being cloned into pMD18 -T Vector,and were compared with the CP gene sequences of TuMV reported in GenBank. The results showed that the CP genes of TuMV - BJ1 and TuMV - BJ2 were all 867 nucleotides and coded 288 amino acids in length, and nucleotids homology was 97.2 % and amino acids homology was 97.6 %. They all shared, with the CP gene sequences of TuMV reported in GenBank,nucleotid homologies of 87.8 % - 97.8 % and amino acid homologies of 91.7 % - 99.0 %. Based on the phylogenetic analysis of the CP gene sequences,TuMV - BJ1 and TuMV - BJ2 belonged to world - B group of TuMV.
出处
《中国蔬菜》
北大核心
2006年第3期6-8,共3页
China Vegetables
基金
国家自然科学基金(30471189)
