慈姑茎尖组织培养与快速繁殖

Tissue Culture and Mass Propagation of Chinese Arrowhead

以慈姑茎尖为外植体,用不同培养基进行组织培养,以所获得的试管苗为材料进行快速繁殖技术研究。结果表明,慈姑茎尖培养的最适启动培养基为MS+6-BA1.0mg·L^(-1)+NAA0.3mg·L^(-1);继代增殖的最适培养基为-S+6-BA3.0mg·L^(-1)+NAA0.1mg·L^(-1),增殖系数为4.35。

The shoot tip of Chinese arrowhead was used as explant,the explants were cultured on the media with different hormones. An effective mass propagation technique had been developed for Chinese arrowhead from shoot tips in vitro. The results showed that the favorable medium for induction was MS ＋6 - BA 1.0 mg · L^-1 ＋ NAA 0.3 mg · L^-1 ;the best medium for regeneration was MS ＋6 - BA 3.0 mg · L^-1 ＋ NAA 0. 1 mg · L^-1 ,the propagation coefficient could up to 4.35.