用RAPD标记检测六种蛇基因组DNA多态性

GENOMIC DNA POLYMORPHISMS IN SIX SPECIES OF SERPENTS USING RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

用 ＲＡＰＤ技术检测了乌梢蛇 Ｚａｏｃｙｓ ｄｈｕｍｎａｄｅｓ、王锦蛇 Ｅｌａｐｈｅ ｃａｒｉｎａｔａ、黑眉锦蛇 Ｅ．ｔａｅｎｉｕｒａ、双斑锦蛇 Ｅ． ｂｉｍａｃｕｌａｔａ、赤链蛇 Ｄｉｎｏｄｏｎ ｒｕｆｏｚｏｎａｔｕｍ和日本蝮短尾亚种 Ａｇｋｉｓｔｒｏｄｏｎ ｂｌｏｍｈｏｆｆｉｉｂｒｅｖｉｃａｕｄｕｓ等６种蛇的基因组ＤＮＡ的多态性。经２０个随机引物扩增得到２５３个多态片段，其中锦蛇属３个种共有片段８条，游蛇科５个种共有片段５条。在这些共享片段中未检测出种内多态现象。片段分布和片段共享度聚类分析所得的游蛇科５种蛇之间的亲缘关系与染色体、颅骨和半阴茎研究的结果基本一致。

Genetic variation in six species of serpents, Elaphe carinata, E. taeniura, E. bimaculata, Zaocys dhumnades, Dinodon rufozonatum and Agkistrodon blomhoffii brevicaudus was detected using randomly amplified polymophic DNA (RAPD) markers. Two hundred and fifty-three amplified fragments were generated by RAPD with 20 primers. Among them, eight fragments were shared by three species of Elaphe and five frag ments by five species of Colubridae. No intraspecific polymorphism was detected in the shared fragments. The relationship indicated by the cluster analysis is consistent with that of the results based on the studies of the karyotypes, skull and hemipenis.