去甲斑蝥酸钠对小鼠卵母细胞第一次减数分裂的影响

EFFECTS OF DISODIUM NORCANTHARIDATE ON THE FIRST MEIOTIC DIVISION OF OOCYTES IN MOUSE

体外培养的小鼠卵母细胞在１２ｈ内可完成第一次减数分裂，排出第一极体。将卵母细胞培养在含２５０μｇ／ｍｌ去甲斑蝥酸钠的培养液中，生发泡破裂（ＧＶＢＤ）过程不受影响，但卵母细胞不能完成减数分裂过程，卵母细胞中没有减数分裂器的形成，染色体紧密凝缩在一起；去甲斑蝥酸钠对小鼠卵母细胞减数分裂的影响在６ｈ内具有可逆性：卵母细胞ＧＶ破裂后用去甲斑蝥酸钠处理２ｈ换正常培养液培养，整个减数分裂过程不受影响；ＧＶ期卵母细胞用去甲斑蝥酸钠连续处理６ｈ，洗去药物继续培养，减数分裂可继续进行，但第一极体的排放时间推迟。去甲斑蝥酸钠对分裂期细胞特异性磷蛋白的出现影响不显著，在连续处理的卵母细胞中分裂期细胞特异性磷蛋白仍然存在。

Oocytes in mouse complete the first meiotic division in 12h when cultured in uitro. Dis odium norcantharidate interferes with the congression and segregation of chromosomes and disrupts completely the meiosis apparatus while it has no effects on GVBD and the appearance of phosphoproteins relevant to mitosis and meiosis. Consequently, oocytes treated with disodium norcantharidate fail to mature and therefore can not extrude the first polar bodies.